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Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry |
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DOI: |
KeyWord:liquid chromatography tandem mass spectrometry urine pantothenic acid biomonitoring |
Author | Institution |
ZHANG Xiao-tao,ZHANG Yun-fei,HOU Hong-wei,CHEN Huan,LIU Yong,WANG An,HU Qing-yuan |
1.国家烟草质量监督检验中心;2.中国科学院合肥物质科学院应用技术所;3.河南省肿瘤医院 |
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Abstract: |
A liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method for the determination of pantothenic acid in urine was developed and validated.After centrifugation and dilution,the sample was separated on an ACPUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm) column produced by the Waters corporation,and ionized in electro spray positive ion mode,then detected using multi reaction monitoring mode.The calibration curve has a good linear range with a correlation coefficient(r) of 0.999 3.The limit of detection is 0.46 ng/mL,the recoveries are in the range of 87.9%-95.3%,and the RSDs are in the range of 2.5%-13.0%.The method has the advantages of high sensitivity and short analysis time,and could be used to analyze pantothenic acid in human urine. |
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