Simultaneous Determination of Uric Acid,Xanthine and Hypoxanthine Based on poly(3-Amino-5-mercapto-1,2,4-triazole)/Multi-walled Carbon Nanotubes Modified Glassy Carbon Electrode

KeyWord:multi-walled carbon nanotubes  3-amino-5-mercapto-1,2,4-triazole  uric acid  xanthine  hypoxanthine  electrochemistry

Abstract:

      In this paper,the poly(3-amino-5-mercapto-1,2,4-triazole)/multi-walled carbon nanotubes modified glassy carbon electrode(pTA/MWCNTs/GCE) was successfully prepared by electrodepositing 3-amino-5-mercapto-1,2,4-triazole(TA) on the MWCNTs/GCE.The electrochemical behaviors of uric acid(UA),xanthine(XA) and hypoxanthine(HX) at the pTA/MWCNTs/GCE were investigated using cyclic voltammetry(CV) and differential pulse voltammetry(DPV).The results showed that the chemically modified electrode displayed excellent electrochemical catalytic activities towards UA,XA and HX.The calibration curves for UA,XA and HX were obtained over the ranges of 9.0-739.0£¬2.0-259.0,1.0-353.0 ¦Ìmol/L with the detection limits of 0.67£¬0.17,0.33 ¦Ìmol/L,respectively.In addition,the modified electrode was applied in the determination of UA,XA and HX in real samples(urine and serum),with the recoveries of the samples ranged between 98.8%-105.5%.