|
Determination of Three Tetracyclines in Pig Kidney by High Performance Liquid Chromatography with Ionic Liquid Dispersive Liquid-Liquid Microextraction |
|
View Full Text Download reader |
DOI: |
KeyWord:ionic liquid(IL) dispersive liquid-liquid microextraction(DLLME) high performance liquid chromatography(HPLC) tetracycline antibiotics(TCs) pig kidney |
Author | Institution |
ZHANG Ying,DING Jian-hua,XIANG Hong-lin,LIU Peng-fei |
东华理工大学化学生物与材料科学学院 |
|
Hits: 1815 |
Download times: 821 |
Abstract: |
A method was established for the determination of three tetracycline antibiotics(TCs) residues such as oxytetracycline,tetracycline and chlortetracycline in pig kidney by high performance liquid chromatography(HPLC) coupled with dispersive liquid-liquid microextraction(DLLME).The influences of dispersant type,amount of ionic liquid,amount of dispersant,pH of sample solution,extraction time and effect of salts on extraction efficiency were investigated.The optimum experimental conditions were as follows:using acetone as the dispersant,a 50 μL ionic liquid([BMIM] PF6),a 140 μL dispersant,a pH 3.0 sample solution,a 15 min of extraction time and no salt.The results showed that this method provided a wide linearity in the range of 0.1-10.0 mg/L with correlation coefficients no less than 0.999 5.The relative standard deviations(RSDs) were 2.2%-3.1%for oxytetracycline,tetracycline and chlortetracycline.The detection limits(LOD) were in the range of 54-93 μg/L with the enrichment folds of 7.0-27.8,and the recoveries were 99.5%-101.1%.It was found that the accuracy and precision could meet the requirements for the rapid,green,sensitive and accurate detection of oxytetracycline,tetracycline and chlortetracycline residues in pig kidney. |
Close |
|
|
|