Determination of Morphine in Urine and Blood by Capillary Electrophoresis Coupled with Indirect Chemiluminescence Detection

KeyWord:chemiluminescence  capillary electrophoresis  morphine  solid phase extraction  urine sample  blood sample

Abstract:

      Based on the fact that morphine has a significant inhibitory effect on Ag(¢ó) complex-luminol chemiluminescence system in alkaline solution and the effect is proportional to the concentration of morphine£¬a novel method was established for the determination of morphine by capillary electrophoresis coupled with indirect chemiluminescence detection.Under the optimized conditions,there was a linear range of 2.0-30.0 mg/L for morphine with a correlation coefficient(r) of 0.999 8 and the detection limit of 0.75 mg/L.The RSD(n=7) was 2.0%for morphine in a concentration of 20 mg/L.Combined with solid phase extraction,the method was successfully applied in the detection of morphine in urine and blood samples with the average recoveries of 109.0%and 101.3%,respectively.With the advantages of high-resolution,sensitivity and low-cost,the method could be used for the determination of morphine in urine and blood samples.