Rapid Determination of Specific Migration of Four Parabens in Food Simulants by HPLC-UV Detection with Organic Phase Anion Exchange Solid Phase Extraction

KeyWord:OPAE  SPE  HPLC-UV  methylparaben  ethylparaben  isopropylparaben  propylparaben  specific migration

Abstract:

      A method for the rapid determination of specific migration(SM) of methylparaben,ethylparaben,isopropylparaben and propylparaben in food simulants(10%ethanol,3%acetic acid,20%ethanol,50%ethanol and olive oil) was developed by HPLC-UV detection with organic phase anion exchange(OPAE) solid phase extraction(SPE).After the migration test,the soaking solution was cooled down,and vortexed.The olive oil food simulant sample was pretreated by organic phase anion exchange solid phase extraction before injection,while the aqueous food simulant(10%ethanol,3%acetic acid,20%ethanol or 50%ethanol solution) sample was just filtered through a polytetrafluoroethylene filter with a disposable syringe before injection.The baseline separations for four parabens were achieved within 12.5 min on a phehyl column.The external standard method for calibration curve was adopted via ultraviolet detector.The linearities of the method for 4 parabens were good with r2¡Ý0.999 8 over the concentration range of 0.5-100 mg/L.The recoveries for 4 parabens in 5 types of food simulants at spiked levels of 10,50,100 mg/kg were between 97.9%and 103%with relative standard deviations of 0.02%-2.2%.The limits of quantitation of the method were between 0.1 mg/kg and 0.5 mg/kg.With the low limits of quantitation,good recoveries and accuracies,the method could meet the requirements of (EU)NO 10/2011 regulation for the specific migration limit of four parabens.The method was applied in the analysis of food contact material samples.