Determination of Four Kinds of Prohibited Aldehyde and Ketone Compounds in Cosmetics by High Performance Liquid Chromatography

KeyWord:high performance liquid chromatography£¨HPLC£©  cosmetics  aldehydes and ketones

Abstract:

      A high performance liquid chromatographic£¨HPLC£© method was established for the simultaneous determination of four kinds of prohibited aldehyde and ketone compounds£¨crotonaldehyde,acetophenone,2,4 dihydroxy 3 methylbenzaldehyde and 2 pentylidene cyclohexanone£© in cosmetics.The samples were extracted with 50% acetonitrile solution,then separated on an AQ-C18£¨4ª±6 mm¡Á150 mm,5 ¦Ìm£© column and detected by HPLC with a diode array detector.The retention time and UV absorption spectra were adopted for qualitation and the external standard method was used for quantitation.The positive cosmetic samples were confirmed by HPLC-MS/MS method.Under the optimal conditions,the results showed that four analytes showed good linearities in the range of 0ª±05-2ª±0 mg/L with correlation coefficients not less than 0ª±999 9.The limits of detection and quantitation were in the ranges of 0ª±5-1ª±0 mg/kg and 1ª±5-3ª±0 mg/kg,respectively.The mean recoveries for the samples at three spiked levels ranged from 89ª±0% to 106ª±7% with the relative standard deviations£¨RSDs,n=6£© of 2ª±0%-6ª±9%.The method is accurate and reliable,and is suitable for the determination of four kinds of prohibited aldehyde and ketone compounds in cosmetics.