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An Impedance Biosensor for Rapid Detection of Alzheimers Marker Protein(Tau) |
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DOI: |
KeyWord:electrochemistry impedance immunity Alzheimer disease(AD) Tau protein |
Author | Institution |
CHEN Yi hong,FAN Kai,LI Du juan,WANG Ming hua |
1.杭州电子科技大学生命信息与仪器工程学院;2.忻州师范学院生物系 |
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Abstract: |
In this paper,a self assembled impedance immune sensor was constructed to detect trace Tau protein in order to achieve the purpose of early diagnosis on Alzheimer disease.The sensor was constructed and characterized by the following steps:the bifunctional molecule 3,3′ dithiobis(sulfosuccinimidylpropionate) (DTSSP) was modified on the gold electrode surface through the Au-S bond,which was then conjugated with antibodies 39E10 through the CO-NH.Tau protein was specifically captured by physisorption using immunoassay,characterized by AC impedance and verified by cyclic voltammetry.By optimizing the DTSSP modification time and antibody concentration,the sensor had a detection signal toward Tau protein of 002-100 μg/mL,in which there existed a good linearity(r2=0988 8) in the concentration range of 02-20 μg/mL with a detection limit of 043 nmol/L.This immunosensors specific capture ability for Tau could be used as a supplementary means for clinical detection on AD. |
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