Determination of Advantame in Foods by High Performance Liquid Chromatography

KeyWord:high performance liquid chromatography£¨HPLC£©  determination  food  advantame

Abstract:

      A method for the determination of advantame in foods using high performance liquid chromatography£¨HPLC£© was developed.The samples were extracted with methanol-water£¨50¡Ã50,by volume£© in ultrasonic condition for 10 min.The supernatant was analyzed after centrifugation.Target compound was separated on an Agilent TC-C18£¨2£©£¨250 mm¡Á4ª±6 mm,5 ¦Ìm£© column at 280 nm,with methanol-0ª±02 mol/L ammonium acetate£¨53¡Ã47,by volume£© as mobile phase by isocratic elution.The quantitative analysis was carried out by the external standard method.The results showed that the calibration curves were linear in the range of 1ª±0-200ª±0 mg/L with correlation coefficients of 0ª±999 8.The limits of detection£¨S/N¡Ý3£© of the method for the analytes in beverage,yoghourt and cake were 0ª±6£¬1ª±5 and 9ª±0 mg/kg,respectively.The average recoveries ranged from 90ª±9% to 103ª±4% with relative standard deviations£¨RSDs,n=5£© of 1ª±1%-4ª±5%.With the advantages of good separation,few impurity disturbances and high accuracy,this method could be applied in the analysis of advantame in real samples.