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Construction and Application of A Room temperature Phosphorescence Sensor for Baicalein |
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DOI: |
KeyWord:quantum dots(QDs) room temperature phosphorescence(RTP) baicalein(BAI) scutellaria baicalensis georgi serum sensor |
Author | Institution |
GUO Yan xia,WANG Yuan yuan,LI Shu xian,WANG Wen ke* |
山西师范大学生命科学学院 |
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Abstract: |
A relatively simple and fast phosphorescence method for the detection of baicalein(BAI) was established,based on that baicalein could quench the room temperature phosphorescence(RTP) of PEI-Mn/ZnS quantum dots(QDs) prepared by water phase coprecipitation through electron transfer effect.The experimental results showed that,at pH 80 and a reaction time of 10 min,the phosphorescence quenching degree of QDs had a linear relationship(r=0997) with BAI in the concentration range of 007-060 mg·L-1.The detection limits of the method were 0039 mg·L-1,and the relative standard deviations were 41%.The spiked recoveries for practical samples were in the range of 960%-1007%.The established method,effectively avoiding the interference of common ions and amino acids etc., could be used for the rapid determination of BAI in scutellaria baicalensis georgi and serum,which is expected to be applied in the determination and analysis of BAI in medicine industry.It also provides a technical basis for the detection of other drugs based on the mechanism of QDs electron transfer. |
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