Determination of Formaldehyde and Acetaldehyde in Cigarette Capsules by High Performance Liquid Chromatography

KeyWord:capsule of cigarette  formaldehyde  acetaldehyde  extraction  high performance liquid chromatography£¨HPLC£©

Abstract:

      A high performance liquid chromatographic£¨HPLC£© method was established for the analysis of formaldehyde and acetaldehyde in cigarette capsules based on a novel extraction tube device integrating extraction£¬filtering and transfer in one step.The cigarette capsules were extracted with aqueous solution of acetonitrile£¨50% by volume£© for 25 min.After purification£¬the extracts were allowed to derivatively react with 2,4 dinitrophenylhydrazine in an acidic condition£¨pH 4ª±8£©.Then the derivatives of the extracts were analyzed by HPLC.The results indicated that the extraction tube device greatly simplified the sample preparation for HPLC analysis.Furthermore£¬the formaldehyde and acetaldehyde in the capsules were successfully separated and determined.The calibration curves for them both were linear in the ranges of 8-1 500 ¦Ìg/L and 10-1 650 ¦Ìg/L£¬respectively£¬with their correlation coefficients£¨r2£© larger than 0ª±999.The limits of detection£¨LOD£© and quantitation£¨LOQ£© for formaldehyde and acetaldehyde were 2ª±5 ¦Ìg/L and 2ª±8 ¦Ìg/L£¬and 8ª±0 ¦Ìg/L and 9ª±5 ¦Ìg/L£¬respectively.The mean recoveries for the target compounds were in the range of 96ª±9%-101ª±1% with the intra day and inter day precisions both below 4%.The method provides a new strategy for the quantitation on formaldehyde and acetaldehyde in cigarette capsules with unprecedented simplicity£¬high precision and great reproducibility.