Determination on Migration Quantity of Four Phenolic Antioxidants in Food Packaging Materials by Gas Chromatography-Mass Spectrometry

KeyWord:phenolic antioxidants  food simulant  food packaging materials  gas chromatography-mass spectrometry£¨GC-MS£©  migration quantity

Abstract:

      A gas chromatography-mass spectrometric£¨GC-MS£© method was established for the determination of migration quantity of 2,6 dimethylphenol£¬p tert butylphenol£¬2,6 di tert butyl 4 methylphenol and butylated hydroxyanisole in food packaging materials£¬with water£¬4%£¨by volume£© aqueous acetic acid£¬50%£¨by volume£© aqueous ethanol and isooctane as the food simulated solution.Samples were soaked with food stimulant£¬and the water based food simulated soaking solution was extracted with ethyl acetate before analysis£¬while the isooctane soaking solution was analyzed without any pretreatment.The separation of target compounds was conducted using a DB-1701 capillary£¨30 m¡Á0ª±25 mm¡Á0ª±25 ¦Ìm£© column.The determination was performed in selective ion mode£¬and external standard method was used for quantitation.The results showed that£¬under optimized conditions£¬there were good linearities for four phenolic antioxidants between peak areas and concentrations in the range of 0ª±1-10 mg/L with correlation coefficients£¨r£© more than 0ª±999.The limits of detection£¨LOD£© of this method were 0ª±01 mg/L£¬and the limits of quantitation£¨LOQ£© were 0ª±03 mg/L.The spiked recoveries for four phenolic antioxidants in fruits at three spiked levels of 0ª±1£¬1ª±0£¬10ª±0 mg/L ranged from 91ª±5% to 110%£¬with relative standard deviations£¨RSDs£¬n=5£© of 1ª±9%-9ª±3%.The method£¬with the advantages of accuracy£¬high reliability and sensitivity£¬was suitable for the determination of migration of four phenolic antioxidants in food packaging materials.