Determination of Nisin A and Nisin Z in Fresh Wet Rice Noodles by High Performance Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:Nisin A  Nisin Z  HPLC-MS/MS  fresh wet rice noodles

Abstract:

      A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method was developed for the simultaneous analysis of Nisin A and Nisin Z in fresh wet rice noodles.An extraction solution of formic acid(pH 3ª±0) was used to extract Nisin A and Nisin Z in fresh wet rice noodles samples.The extract was separated on a Hilic column,and determined with electrospray ionization(ESI) source in positive ion mode under multiple reaction monitoring(MRM) mode.There were good linear relationships for the analytes between their peak area responses and concentrations of 100-2 000 ¦Ìg/L,with correlative coefficients larger than 0ª±999.The limits of detection for Nisin A and Nisin Z were 0ª±01 mg/kg and 0ª±02 mg/kg,respectively.The recoveries were in the range of 95ª±6%-107ª±0% with RSDs less than 2ª±0%.The method was reliable and accurate,and was suitable for the determination of Nisin A and Nisin Z in fresh wet rice noodles.