Determination of Spinetoram Residues in Radix Gotonopsic Tongshen Otiv.Using Ultra high Performance Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:spinetoram  ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)  Radix Gotonopsic Tongshen Otiv.  residues determination

Abstract:

      An ultra high performance liquid chromatography-tandem mass spectrometric(UHPLC-MS/MS) method was established for the determination of spinetoram residues,XDE-175-J and XDE-175-L in Radix Gotonopsic Tongshen Otiv.The samples were exacted with acetonitrile,and purified with primary secondary amine and graphitized carbon black.The target compounds were quantified by the external standard method.There were good linearities for the peak areas of XDE-175-J and XDE-175-L in the concentration ranges of 0ª±075-75 ¦Ìg/L and 0ª±025-25 ¦Ìg/L,respectively.The average recoveries for XDE-175-J and XDE-175-L at the spiked levels of 0ª±375,3ª±75,75 ¦Ìg/kg and 0ª±125,1ª±25,25 ¦Ìg/kg were in the ranges of 88ª±4%-113ª±5% and 84ª±4%-99ª±5%,with relative standard deviations(RSDs) of 2ª±0%-4ª±2% and 2ª±5%-4ª±9%,respectively.The limits of quantitation(LOQs) for XDE-175-J and XDE-175-L were 0ª±375 ¦Ìg/kg and 0ª±125 ¦Ìg/kg,respectively.With the advantages of simplicity,rapidness and convenience,the method could meet the requirements for pesticide residues analysis,and be applied in the rapid detection of spinetoram residues in Radix Gotonopsic Tongshen Otiv.