Determination of Three Xylene Isomers in Seafoods by GC-MS/MS Method with Headspace Injection

KeyWord:headspace  gas chromatography-triple quadrupole mass spectrometry£¨GC-MS/MS£©  xylene  isomer  seafood

Abstract:

      A method based on gas chromatography-triple quadrupole mass spectrometry with headspace£¨HS/GC-MS/MS£© was developed for the determination of three xylene isomers in seafoods.The samples were incubated at 90 ¡æ and oscillated for 40 min,then separated on an HP-INNOWax column.The determination of the target compounds were performed by GC-MS/MS under the multiple reaction monitoring£¨MRM£© mode,and the quantification was carried out using the external standard method.The calibration curves for three xylene isomers were linear in the range of 5ª±0-100ª±0 ¦Ìg/kg with their correlation coefficients£¨r2£© larger than 0ª±99.The limits of detection£¨LOD£© and quantitation£¨LOQ£© of the method were 2ª±5 ¦Ìg/kg and 5ª±0 ¦Ìg/kg,respectively.The recoveries at three spiked level of 6ª±0,15ª±0,50ª±0 ¦Ìg/kg were in the range of 89ª±6%-112ª±4% with relative standard deviations£¨RSD£© not more than 11ª±5%.The method was successfully applied in the determination of xylene isomers in seafoods.With the advantages of simple operation,accuracy and high sensitivity,the method was suitable for the determination of xylene residues in seafoods.