Determination of 10 Antiviral Drugs in Surface Water by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction

KeyWord:ultra performance liquid chromatography-tandem mass spectrometry£¨UPLC-MS/MS£©  antiviral drugs  surface water  solid phase extraction£¨SPE£©

Abstract:

      An ultra performance liquid chromatography-tandem mass spectrometric£¨UPLC-MS/MS£© method was developed for the simultaneous analysis of 10 antiviral drugs,eª±g.valaciclovir,abacavir,entecavir,zidovudine,nevirapine,ganciclovir,telbivudine,lamivudine,acyclovir and amantadine in surface water.The aqueous samples were concentrated and purified on an ISOLUTE ENV+ cartridge,and then separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution with 0ª±1% formic acid-acetonitrile as mobile phase.The detection of antiviral drugs was performed by tandem mass spectrometry in electrospray ionization under multiple reactions monitoring£¨MRM£© mode.The calibration curves for target compounds were linear over the range of 0ª±5-200 ng/L with correlation coefficients£¨r2£© larger than 0ª±993.The average recoveries for antiviral drugs in surface water at three spiked concentration levels ranged from 70ª±0% to 104ª±0%,with the limits of quantitation£¨LOQ£© of 0ª±2-5ª±0 ng/L and the relative standard deviations£¨RSDs£© less than 15%.This method could be applied in the analysis of antiviral drugs in aqueous samples.