Determination of 187 Pesticide Residues in Dried Persimmon by Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£©  positive electrospray ion source  dried persimmon  pesticide multi residue

Abstract:

      A method for the determination of 187 kinds of pesticides in dried persimmon by liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£© was established.The samples were extracted with acetonitrile containing 1% acetic acid£¬then purified with anhydrous magnesium sulfate£¬anhydrous sodium acetate£¬anhydrous sodium sulfate£¬N propyl ethylenediamine£¨PSA£© and C18.The separation of targeted compounds was performed on a ZORBAX Eclipse Plus-C18 column by gradient elution using 95% acetonitrile aqueous solution£¨organic phase£© and water£¨aqueous phase£© as mobile phases£¬both contained 0ª±005 mol/L ammonium formate and 0ª±01% formic acid.The compounds were detected by LC-MS/MS with positive electrospray ion source£¨ESI+£© under dynamic multiple reaction monitoring£¨DMRM£© mode.The matrix-matched external standard method was used for the quantitation.The callibration curves for 187 pesticides were linear in the range of 1-100 ¦Ìg/kg with correlation coefficients£¨r2£© more than 0ª±99.The limits of detection£¨S/N¡Ý3£© and quantitation£¨S/N¡Ý10£© for the analytes were 1 ¦Ìg/kg and 10 ¦Ìg/kg£¬respectively.The recoveries were in the range of 70ª±2%-118% with relative standard deviations£¨RSDs£¬n=6£© of 2ª±0%-20%.The method is suitable for the high throughput detection on pesticide multi residues in dried persimmon with its advantages of accuracy£¬sensitivity and rapidness.