Determination of Aflatoxins in Edible Oil by High Performance Liquid Chromatography with Stirring Bar Sorptive Extraction Based on Dopamine-Graphene Oxide Complex

KeyWord:mussel-bioinspired chemistry  dopamine-graphene oxide complex  stirring bar sorptive extraction£¨SBSE£©  high performance liquid chromatography with fluorescence detector£¨HPLC/FLD£©  edible oil  aflatoxin £¨AF£©

Abstract:

      A method of stirring bar sorptive extraction£¨SBSE£© based on mussel-bioinspired chemistry coating and high performance liquid chromatography with fluorescence detector£¨HPLC/FLD£© was developed for the simultaneous determination of aflatoxin B1£¬B2£¬G1 and G2£¨AFB1£¬AFB2£¬AFG1 and AFG2£© in edible oil.The sample was extracted by SBSE with dopamine-graphene oxide complex as solid phase extraction material prepared by mussel-bioinspired chemistry.The target compounds were separated on a Kromasil C18 column using methanol-acetonitrile-water aqueous solution£¨3¡Ã3¡Ã5 by volume£¬adjust pH 3ª±5 with 10%phosphoric acid£© as mobile phases£¬and detected with a fluorescence detector.The calibration curves for AFB1£¬AFB2£¬AFG1 and AFG2 were linear in the concentration range of 0ª±200-10ª±0 ¦Ìg/L£¬with their correlation coefficients£¨r2£© not less than 0ª±998 9.The spkied recoveries for 4 aflatoxins£¨AFs£© in edible oil samples ranged from 81ª±5% to 96ª±9%£¬with intra day relative standard deviations£¨RSDs£© of 1ª±7%-3ª±4% and inter day RSDs of 1ª±9%-3ª±5%.The limits of detection£¨LODs£© were in the range of 0ª±025-0ª±050 ¦Ìg/L.The proposed method is efficient£¬sensitive and reliable£¬and is suitable for the determination of AFB1£¬AFB2£¬AFG1 and AFG2 in edible oil.