Determination of Migration of 5 Ethanolamine Compounds in Food Contact Materials by Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:food contact material  ethanolamine compounds  liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£©  migration

Abstract:

      A method was developed for the determination of migration of 5 ethanolamine compounds in food contact materials by liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£©.Samples were soaked with five food simulants£¨water£¬4% acetic acid£¬10% ethanol£¬50% ethanol£¬95% ethanol£©.4% acetic acid soaking solution was dried out with nitrogen£¬and then rehydrated with water.Other soaking solutions were injected directly after filtration with membrane.The separation of target compounds was performed with an amino column BEH Amide£¨50 mm¡Á2ª±1 mm£¬1ª±7 ¦Ìm£©£¬using acetonitrile-5 mmol/L ammonium acetate as mobile phase by gradient elution.The detection on the analyte was performed with positive electrospray ionization source under multiple reaction monitoring£¨MRM£© mode£¬and the quantitation was carried out by external standard method.Under the optimized conditions£¬the calibration curves were linear in the range of 1ª±0-750ª±0 ¦Ìg/L£¬with correlation coefficients larger than 0ª±997.The limits of detection£¨LODs£¬S/N=3£© and quantitation£¨LOQs£¬S/N=10£© were in the ranges of 0ª±3-5ª±0 ¦Ìg/kg and 1ª±0-15ª±0 ¦Ìg/kg£¬respectively.The average recoveries for 5 ethanolamine compounds at the spiked levels of 1ª±0-750ª±0 ¦Ìg/kg were in the range of 90ª±2%-118%£¬with relative standard deviations of 0ª±5%-8ª±8%.The method is sensitive and accurate£¬and could meet the requirements for correlative determination.