Determination of Ginsenosides Rg1,Re and Rb1 in Chinese Herbal Residues by HPLC

KeyWord:ginsenosides  high performance liquid chromatography£¨HPLC£©  Chinese herbal residues

Abstract:

      A fast and sensitive reversed high performance liquid chromatographic£¨HPLC£© method was developed for the simultaneous detection of three ginsenosides,iª±e.Rg1,Re and Rb1 in Chinese herbal residues.Effects of solvent,temperature,solvent matrix ratio and time on extraction efficiency were investigated.The sample was ultrasonically extracted with 10 times liquid of 70% methanol at 40 ¡æ for 0ª±5 h.Chromatographic separation was performed on a Zorbax SB-C18£¨4ª±6 mm¡Á150 mm,5 ¦Ìm£© column by gradient elution using water-acetonitrile as mobile phase at a flow rate of 1ª±0 mL/min.The injection volume was 20 ¦ÌL,the column temperature was 20 ¡æ and the detection wavelength was 203 nm.External calibration of peak area versus concentration was used for the quantitation.There were excellent linearities for ginsenosides Rg1£¬Re and Rb1 in the ranges of 18ª±74-257ª±61,37ª±48-468ª±54 and 3ª±88-96ª±93 mg/L,respectively£¬with their correlation coefficients all above 0ª±999.The limits of quantitation£¨LOQ,S/N=10£© for Rg1,Re and Rb1 were 17ª±69,13ª±94 and 1ª±14 mg/L,respectively.The average recoveries at three spiked levels were in the range of 89ª±1%-107%,with relative standard deviations£¨RSD,n=3£© of 0ª±6%-4ª±1%.The method was applied in the detection of ginsenosides Rg1£¬Re and Rg1 in Chinese herbal samples with simplicity,rapidness and high sensitivity.