Determination of Nucleotides in Foods and Milk Products for Infants and Young Children by Anion Exchange High Performance Liquid Chromatography

KeyWord:high performance liquid chromatography£¨HPLC£©  strong anion exchange£¨SAX£©  nucleotides  matrix interference  infants and young children  food and milk products

Abstract:

      A method of anion exchange high performance liquid chromatography was developed for the determination of five nucleotides£¬including cytidine monophosphate(CMP)£¬adenosine monophosphate(AMP)£¬uridine monophosphate(UMP)£¬guanosine monophosphate(GMP) and inosine monophosphate(IMP).The samples were extracted with water£¬then precipitated protein with precipitants.The determination of analytes was performed by HPLC on a Sax column using methanol-KH2PO4£¨10 mmol/L£¬pH 4ª±5£© as mobile phase by gradient elution.The detection wavelength was set at 254 nm£¬and the external standard method was used for quantitation.Result showed that there were good linearities for five nucleotides in the concentration range of 0ª±200-200 mg/L£¬with their correlation coefficients£¨r£© of 0ª±999 8-1ª±000 0.The spiked recoveries were in the range of 95ª±5%-108% with RSDs of 1ª±0%-3ª±2%.With the advantages of good separation£¬wide linear range£¬good stability and accuracy£¬the method solved the matrix interference problem in sample detection based on GB 5413ª±40-2016 5ª±2£¬and enriched the chromatographic conditions for GB 5413ª±40-2016.