Study on Degradation of Flour Additive Azodicarbazide to Semicarbazide under Heat Treatment

KeyWord:high performance liquid chromatography-tandem mass spectrometry£¨HPLC-MS/MS£©  azodicarbazide  semicarbazide  heat treatment

Abstract:

      A method was developed for the determination of semicarbazide£¨SEM£© by high performance liquid chromatography-tandem mass spectrometry£¨HPLC-MS/MS£©.The degradation of azodicarbazide£¨ADA£© to SEM under heat treatment was also studied.The method was optimized by using 2 nitrobenzaldehyde as derivative agent£¬at a derivative temperature of 37 ¡æ and an extraction pH value of 7ª±0.The calibration curve was linear in the range of 0ª±5-100 ¦Ìg¡¤L-1 with a correlation coefficient£¨r£© of 0ª±999 and a detection limit of 0ª±4 ¦Ìg¡¤L-1ª±The spiked recoveries at three spiked levels of 10£¬20 and 40 ¦Ìg¡¤L-1 were in the range of 99ª±6%-120%with relative standard deviations less than 7.6%.Results showed that ADA was degraded into SEM under heat treatment£¬and it was degraded completely at high temperature.Meanwhile£¬the formation rate of SEM increased with the increase of temperature£¬and it reached the maximum value of 0ª±094% at 200 ¡æ.Nevertheless£¬the formation rate decreased with the decomposition of SEM when the temperature was exorbitant£¨>200 ¡æ£©.Besides£¬it also decreased when the baking time lasted too long£¨>30 min£©.