Construction of Huperzine A Immunosensor with Graphene-Chitosan Modified Electrode
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KeyWord:graphene  chitosan  huperzine A  electrochemical immunosensor  differential pulse voltammetry(DPV)
CHEN Yuan-yuan , GAO Xuan, LIU Yuan-yuan, YOU Jin-kun, ZHANG Hong-yan, YU Yu-yan 福建中医药大学药学院
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      A high-sensitive electrochemical immunosensor was prepared by immobilizing anti-HupA monoclonal antibody onto the glassy carbon electrode modified with a reduced graphene oxide/chitosan composite membrane.And an electrochemical immunosensor for the detection of huperzine A(HupA)was established as the antigen-anibody reaction could block the electron transfer of[Fe(CN)6]3-/4- redox system.The electrochemical behavior of HupA in[Fe(CN)6]3-/4- redox system was investigated by cyclic voltammetry(CV),electrochemical impedance spectroscopy(EIS)and differential pulse voltammetry(DPV).Under the optimized conditions, there existed a good linearity between HupA concentration logarithm and the difference of peak current in the assay system of 15 mmol/L K3Fe(CN)6+0.1 mol/L KCl+0.1 mol/L PBS(pH 6.5) in range of 1.0×10-13-1.0×10-10 mol/L with a correlation coefficient(r)of 1.000 0.The detection limit was 3.0×10-14 mol/L, and the recoveries were in the range of 99.4%-102%.With the characteristics of strong specificity, high sensitivity, simple operation and environmental protection, this method could be used for the rapid detection of huperzine A in natural herbs.