Fluorescent Recognition of Metal Ions in Water Using Dimethyl Curcumin Liposome
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KeyWord:dimethyl curcumin(ASC-J9)  liposome  fluorescence probe  metal ion  water
LI Zheng-yi,SHAO Dan-di,YIN Yue,XIAO Tang-xin,SUN Xiao-qiang 常州大学石油化工学院
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      A water-soluble molecular fluorescence probe,dimethyl curcumin(ASC-J9)liposome was developed for the recognition of Fe3+,Fe2+ and Cu2+ through fluorescence quenching.The ASC-J9 liposome was prepared by thin-film dispersion,which had a uniform particle size distribution with a particle size of 145.7 nm and a polymer dispersity index(PDI)of 0.361.The optimal fluorescence conditions obtained by conditional screening were as follows:ASC-J9 liposome concentration:5.0×10-5 mol/L,balance time:5 min,temperature:25 ℃.The binding modes of ASC-J9 liposome with Fe3+,Cu2+ and Fe2+ were achieved by Jobs plot and fluorescence titration.Results showed that the binding ratios for the probe to Fe3+ and Cu2+ were both 1∶1,while that for the probe to Fe2+ was 2∶1.The fluorescence quenching type was determined by Stern-Volmer plot as static quenching,and the association constants of KFe(Ⅲ),KFe(Ⅱ)and KCu(Ⅱ)were 9.63×104 L/mol,3.65×105 L/mol and 2.32×105 L/mol,respectively.With the advantages of rapid detection and high sensitivity,this method was applied in the identification of Fe3+,Fe2+ and Cu2+ ions in water samples.The calibration curves for Fe3+,Fe2+ and Cu2+ were 5.0×10-7 -3.0×10-5 mol/L,5.0×10-7 -1.75×10-5 mol/L and 5.0×10-7 -2.5×10-5 mol/L,with their limits of detection of 6.41×10-7 mol/L,3.28×10-7 mol/L and 5.08×10-7 mol/L,respectively.