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Application Progress of CRISPR/Cas in Nucleic Acid Detection |
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DOI: |
KeyWord:CRISPR Cas proteins specific recognition collateral cleavage nucleic acid detection |
Author | Institution |
SHI Kai,LEI Chun yang,NIE Zhou |
湖南大学化学生物传感与计量学国家重点实验室 |
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Abstract: |
Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)is an adaptive immune defense system based on RNA for most bacteria and archaea.CRISPR/Cas technology modified from CRISPR/Cas system has become a strong genome editing tool,which has been widely applied in gene function analysis,gene modification and therapy.Other than being a strong genome editing tool,the “collateral cleavage” of type Ⅱ Cas has been employed as a rapid,inexpensive,and sensitive nucleic acid detection tool,exhibiting a great promise in nucleic acid molecular diagnosis.The representative research progresses in nucleic acid detection based on the type Ⅱ Cas are reviewed in this article,and an outlook on the application of CRISPR/Cas system in molecular diagnosis is proposed. |
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