Simultaneous Determination of 17 Ginsenosides in Different Stored Products of Ginseng Radix et Rhizoma Rubra by UFLC-QTRAP-MS/MS

KeyWord:Ginseng Radix et Rhizoma Rubra  ultra fast liquid chromatography tandem triple quadrupole mass spectrometry£¨UFLC-QTRAP-MS/MS£©  ginsenosides  storage conditions  principal component analysis£¨PCA£©  hierarchical cluster analysis£¨HCA£©

Abstract:

      A comprehensive analytical method based on ultra fast liquid chromatography tandem triple quadrupole mass spectrometry£¨UFLC-QTRAP-MS/MS£© was developed for the simultaneous determination of 17 ginsenosides in different stored samples of Ginseng Radixet Rhizoma Rubra.Under the optimized chromatographic conditions£¬good separation for seventeen target compounds were obtained on a SynergiTM Hydro-RP 100 A column£¨100 mm¡Á2.0 mm£¬2.5 ¦Ìm£© using 0.1%aqueous formic acid-acetonitrile as mobile phase at a flow rate of 0.4 mL/min.The target compounds were analyzed under multiple reaction monitoring£¨MRM£© mode with an ESI source operating in negative ion mode£¬hierarchical cluster analysis£¨HCA£© and principal component analysis£¨PCA£© were used for data processing.The results showed that there existed good linearities for the 17 components in the certain concentration ranges£¬with their correlation coefficients larger than 0.999 0.The precision£¬repeatability and stability of the method were all satisfactory.The spiked recoveries for the compounds were between 96.4%and 103%£¬with relative standard deviations less than 5%.The results showed that the optimum storage conditions for Ginseng Radixet Rhizoma Rubra were as follows£ºstoring it with a carton at -20 ¡æ£¬protected from light.The proposed method is useful for assessment and control on the quality of Ginseng Radix et Rhizoma Rubra£¬and provides a scientific basis for choosing the most suitable storage condition of Ginseng Radix et Rhizoma Rubra.