Determination of Carnosine and Anserine in Animal derived Food Extracts by UHPLC-ESI-MS/MS

KeyWord:ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-ESI-MS/MS)  carnosine  anserine  animal derived food extract

Abstract:

      A method was established for the simultaneous determination of carnosine and anserine in animal derived food extracts by ultrahigh performance liquid chromatography-electrospray ionization triple quadrupole tandem mass spectrometry.The separation on the analytes was performed by an ultrahigh performance liquid separation system with an Inertsil Amide HP(2.1 mm¡Á100 mm,3 ¦Ìm) column using 0.1% acetic acid and acetonitrile as mobile phases by gradient elution,and the external standard method of peak area was used for quantitation.There existed good linear relationships for the peak areas of carnosine and anserine in the concentration range of 1.95-500 ¦Ìg/L,with their correlation coefficients(r) larger than 0ª±998.The detection limits for carnosine and anserine were 0.12 ¦Ìg/L and 0.47 ¦Ìg/L,respectively.The low concentration oligopeptide raw material was used as the substrate,and the quality control of 2,20,and 200 ¦Ìg/L was spiked.The recoveries for carnosine and anserine at three spiked levels of 2,20 and 200 ¦Ìg/L were in the ranges of 99ª±3%-109% and 100%-113%,with their relative standard deviations(RSD,n=6) of 0.98%-1.1% and 1.0%-1.1%,respectively.With the advantages of simple pretreatment,rapidness,good reproducibility and high precision,the method is applicable for the simultaneous detection of carnosine and anserine in animal derived food extracts.