| A novel benzenesulfonate silica based mixed mode stationary phase with double ligand was prepared by thiol ene click chemistry method.Both 13C NMR and elemental analysis data showed that the phenyl and benzenesulfonate were successfully bonded to the surface of stationary phase.Depending on solute structure and composition of mobile phase,the mixed mode stationary phase provided different separation modes,including reversed phase（RP）,hydrophilic（HILIC） and strong cation exchange（SCX） interactions.The RP capability achieved the separation of alkylbenzenes with good methylene selectivity.The HILIC behavior was evaluated by employing several nucleosides and bases.Moreover,retention factors of solutes versus volume percentage of aqua in mobile phase exhibited a typical U shaped curve,which indicated that Ph/BS phase has RP and HILIC dual retention mechanism.The retention behavior of amine compounds on the stationary phase reflected RP and SCX mixed mode retention characteristic.In addition,the effective separation of melamine and related purities was obtained in SCX mode.Acidic,neutral and basic drugs were also well separated under the RP and SCX mixed mode conditions.This mixed mode stationary phase has the potential advantages in the separation analysis of complex samples. |