Study of Rapid Classification Model of Sandalwoods Based on Proton Nuclear Magnetic Resonance and Partial Least Squares-Discriminant Analysis

KeyWord:sandalwood  nuclear magnetic resonance£¨NMR£©  pattern recognition  partial least squares-discriminant analysis£¨PLS-DA£©

Abstract:

      A rapid method for the identification of sandalwoods was developed based on proton nuclear magnetic resonance£¨1H NMR£©with pattern recognition such as clustering analysis£¬principal component analysis£¨PCA£©and partial least squares-discriminant analysis£¨PLS-DA£©to build the models of identification.The samples included 6 species of sandalwood£¬i.e.Dalbergia oliveri£¬Dalbergia bariensis£¬Dalbergia cochinchinensis£¬Pterocarpus erinaceus£¬Pterocarpus macrocarpus and Pterocarpus santalinus£¬and 2 adulterants of sandalwood such as Gluta sp.and Pterocarpus tinctorius.After ultrasonic treatment in MeOD£¬the extracts were determined by 1H NMR.Results showed that the contribution of first three principal components derived from the PLS-DA model was added up to 79%.Except that group Dalbergia cochinchinensis interacted with group Dalbergia oliveri and group Pterocarpus santalinus£¬respectively£¬the other groups could be separated from each other.Obviously£¬the adulterants could be easily distinguished from the sandalwoods by this method.Therefore£¬the method was rapid and high sensitive in the distinguishment of adulterants£¬and showed a great potential in the identification of different species of sandalwood.