Determination of 7 Mycotoxins in Shrimp Feed by Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:mycotoxin  liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£©  feed

Abstract:

      A liquid chromatography-tandem mass spectrometric£¨LC-MS/MS£© method was developed for the quantitative determination of aflatoxin B1£¨AFB1£©,aflatoxin M1£¨AFM1£©,T-2 toxin£¨T-2£©,HT-2 toxin£¨HT-2£©,deoxynivalenol£¨DON£©,ochratoxin A £¨OTA£© and zearalenone£¨ZEN£© in shrimp feed.Samples were extracted with 15 mL acetonitrile-water£¨4¡Ã1,by volume£©,and degreased with 10 mL acetonitrile saturated hexane.The extract was cleaned up with a MycoSpinTM400 multitoxin LC-MS/MS clean-up column.The target compounds were separated on an Hypersil Gold£¨150 mm¡Á2.1 mm,5 ¦Ìm£©chromatographic column by gradient elution with methanol-water containing 0.03%ammonia as mobile phase,and analyzed simultaneously by electron spray ionization mass spectrometry in positive or negative ion mode,and quantified by the matrix-matched external standard method.Under the optimized conditions,there were good linearities for the analytes in corresponding concentration range with their correlation coefficients£¨r2£© more than 0.991.The limits of detection£¨LOD£©ranged from 1.83 ¦Ìg/kg to 12.63 ¦Ìg/kg.Average recoveries for the target mycotoxins at high,medium and low spiked levels ranged from 87.5%to 116%with relative standard deviations of 2.4%-18%.The developed method could provide a reference for the simultaneous determination of mycotoxins in aquatic feeds.