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| A Novel Method for Purification of the Recombinant Human Serum Albumin from Transgenic Pig Plasma |
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| DOI： |
| KeyWord:recombinant human serum albumin(rHSA) purification thermal ethanol precipitation multi-stage liquid chromatographic techniques |
| Author | Institution |
| YU Qian，WANG Yong，QIAN Xiao-hong，QIN Wei-jie，ZHANG Pu-min，ZAHNG Yang-jun |
1.Graduate School,Anhui Medical University;2.State Key Laboratory of Proteomics,Beijing Proteome Research Center,Beijing Institute of Lifeomics,Military Medical Science Academy of the PLA;3.Department of Laboratory Animal Science,College of Basic Medical Sciences,Army Medical UniversityThird Military Medical University |
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| Abstract: |
| Gene engineering technology has become a key technique for research and production of recombinant human serum albumin(rHSA) replacing human serum albumin(HSA),while the methods for rHSA purification are the key point to this technique.A novel method for rHSA purification from transgenic pig plasma was introduced in this paper,in which thermal ethanol precipitation combined with multi stage liquid chromatography was chosen for rHSA purification.The rHSA was firstly extracted from pig plasma by the modified thermal ethanol precipitation method,with its purity and recovery of 69.5% and 51.3%,respectively.Then,the crude extract was further purified by multi stage liquid chromatographic techniques,i.e.anion exchange chromatography and reversed phase chromatography,with the purity and recovery for rHSA actually up to 100.0% and 41.1%,respectively.This method provides an opportunity for the large scale purification of rHSA from transgenic pig plasma for clinical and biochemical research,and lays a foundation for the application of rHSA in place of HSA. |
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