| An ultra performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry(UPLC-Q-Orbitrap HRMS) with data dependent acquisition mode was developed for the analysis of 15 functional ingredients in food supplements of pueraria,and a corresponding standard library was also established.By studying the fragmentation mechanism of the functional ingredients,the fragments during fragmentation were obtained.Data-independent acquisition mode was used for the rapid screening of known and unknown functional ingredients in food supplements of pueraria.There existed good linear relationships for 15 functional components in corresponding concentration ranges with correlation coefficients(r2) larger than 0.99.The recoveries at different spiked levels ranged from 83% to 110%,with relative standard deviations(RSD) of 2.1%-8.6%.The established method was applied in actual sample analysis,in which 16 components were screened out,and 4 of them were components beyond the established standard library.Results indicated that the method was feasible in the screening of functional components in food supplements of pueraria. |