Detection of Quercetin Using a Fluorescence Probe Based on Organosilane-functionalized Carbon Dots

KeyWord:organosilane functionalized carbon dots(SiCDs)  fluorescence quenching  nanoprobe  quercetin

Abstract:

      A simple and efficient method for the detection of quercetin was established based on the fluorescence quenching effect of quercetin toward organosilane functionalized carbon dots(SiCDs).The highly luminescent SiCDs were prepared through one step hydrothermal method,with citric acid as carbon source and organosilane as surface coating agent.The as synthesized SiCDs showed a strong emission at 450 nm when excited at 360 nm,and the fluorescence quantum yield was up to 69ª±2%.Effects of action time,pH value and carbon dots concentration on the detection of quercetin were investigated.Furthermore,the fluorescence quenching mechanism was elucidated.Under the optimal conditions,a linear fluorescence signal response to quercetin concentration was obtained in the range of 1ª±0-40ª±0 ¦Ìmol/L with a correlation coefficient(r) of 0ª±996 7,and the detection limit was 3ª±8 nmol/L.The method was applied in the detection of quercetin in practical samples with satisfactory results.