Simultaneous Detection of 8 Components in Hedera Helix L by Ultra High Performance Liquid Chromatography

KeyWord:Hedera helix L.  ultra high performance liquid chromatography(UHPLC)  ivy saponins  organic acids  flavonoids

Abstract:

      A method of ultra high performance liquid chromatography(UHPLC) was developed for the simultaneous determination of eight components,iª±e.chlorogenic acid,cryptochlorogenic acid,rutin,nicotifiorin,hederacoside C,hederacoside D,hederasaponin B and ¦Á hederin in Hedera helix L..The dry leaves passed through 40 mesh sieve were put in 80% methanol with solid-liquid ratio of 1¡Ã100.The solution then was refluxed by water bath for 1 h at 85 ¡æ to prepare the test solution.The chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus-C18(2.1 mm¡Á100 mm,1.8 ¦Ìm) column by gradient elution with acetonitrile and 0.05%phosphoric acid as mobile phases.Results indicated that the eight components in Hedera helix L.were well separated,and the relative standard deviations(RSD) of the method for precision and reproducibility were less than 3.0%.The eight components were found to be stable for 24 h in room temperature,and there existed good linear relationships for the analytes in corresponding mass concentration ranges with their correlation coefficients(r) not less than 0.999 5.The limits of detection and the limits of quantitation were in the ranges of 0.40-10.58 ¦Ìg¡¤mL-1 and 1.31-34.61 ¦Ìg¡¤mL-1,respectively.Average recoveries for the 8 components were between 97.3%and 108%with RSD(n=6) of 0.51%-3.2%.Therefore,this method could be applied in the quantitative analysis of the 8 components in Hedera helix L..