Simultaneous Determination of Lysophosphatidylcholines in Biological Samples by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

KeyWord:Fourier transform ion cyclotron resonance mass spectrometry(FTICR-MS)  lysophosphatidylcholines(LPCs)  biological samples

Abstract:

      A Fourier transform ion cyclotron resonance mass spectrometric(FTICR-MS) method was developed for the simultaneous and rapid determination of four lysophosphatidylcholine(LPCs) in complex biological samples.The biological sample was ultrasonically extracted with methanol-chloroform(9¡Ã 1,by volume) for 30 min,and filtered with a 0.22 ¦Ìm filter membrane for analysis.FTICR-MS determination were performed in direct infusion injection mode,using a 250 ¦ÌL microsampler at a flow rate of 120 ¦ÌL/h.MS spectra were recorded in a mass to charge ratio(m/z) range of 50-1 000 under positive ion electrospray ionization(ESI) mode.Quantitative analysis was conducted by the external standard method.Results indicated that the four investigated LPCs showed good linearity in the concentration range of 0.5-100 ¦Ìg/L,with correlation coefficients(r2) not less than 0.993 0.The limits of detection and quantitation were in the ranges of 0.02-0.03 ¦Ìg/L and 0.07-0.1 ¦Ìg/L,respectively.The average recoveries of four LPCs from plasma and rat liver samples at three spiked levels ranged from 70.8% to 95.0%,with relative standard deviations(RSD) of 1.2%-9ª±8%.The developed method showed the advantages of simplicity,rapidness,sensitivity,accuracy and good repeatability,and was suitable for the rapid determination of LPCs in complex biological samples.