Simultaneous Determination of 20 Flavonoid Glycosides and Terpenoid Lactones in Shuxuening Injection by Ultra high Performance Liquid Chromatography-Mass Spectrometry

KeyWord:shuxuening injection  ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)  flavonoid glycosides  terpenoid lactones  simultaneous quantitative analysis

Abstract:

      A method of ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 16 flavonoid glycosides and 4 terpenoid lactones in shuxuening injections.The samples were firstly diluted with methanol water(1%1,by volume),and then separated on an ACQUITY UPLC BEH Shield RP18 column(1ª±7 ¦Ìm,2ª±1 mm¡Á100 mm) by gradient elution using acetonitrile-water containing 0ª±1% formic acid as mobile phases,finally detected by UPLC-MS/MS with ESI source in MRM mode.Results revealed that the 20 compounds could be analyzed within 10 min.There were good linear relationships for twenty compounds in a certain concentration range with their correlation coefficients(r2) no less than 0ª±963 3.The limits of detection(LODs) and limits of quantitation(LOQs) were in the ranges of 0ª±02-1ª±59 ng/mL and 0ª±07-5ª±30 ng/mL,respectively.And the recoveries at three levels were between 85ª±9-109%.This method was applied in the simultaneous detection of 16 flavonoid glycosides and 4 terpenoid lactones in different shuxuening injection products.With the advantages of simplicity,rapidness and good repeatability,the method is suitable for the quantitative analysis of 20 compounds in shuxuening injections,and provides a good strategy for further quality control of shuxuening injections.