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| Screening of α Glucosidase Inhibitors in Scutellaria Baicalensis by Liquid Chromatography-Mass Spectrometry with Microdialysis |
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| DOI： |
| KeyWord:Scutellaria baicalensis α glucosidase microdialysis HPLC-MSn inhibitor |
| Author | Institution |
| PANG Bo，WANG Qian-qian，LIU Shu，LIU Zhi-qiang，SONG Feng-rui |
1.National Center of Mass Spectrometry in Changchun,Key Laboratory of Traditional Chinese Medicine Chemistry and Mass Spectrometry Jilin Province,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,China;2.School of Applied Chemistry and Engineering,University of Science and Technology for China,Hefei,China;3.State Key Laboratory of Medicinal Chemical Biology,Nankai University,Tianjin,China |
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| Abstract: |
| A liquid chromatography-mass spectrometry with microdialysis was used to screen potential α glucosidase inhibitors in the extracts of Scutellaria baicalensis and identify the structures of those bioactive components screened.The chromatographic separation was carried out on a Dikma C18 column(150 mm×46 mm,5 μm) by gradient elution with 05% acetic acid solution-acetonitrile as mobile phases at a flow rate of 05 mL/min and a column temperature of 30 ℃.Mass spectrometry analysis and data acquisition were conducted in the positive ion mode.In this work,ten potential α glucosidase inhibitors in Scutellaria baicalensis were found and identified simultaneously.The binding degrees of 10 compounds with α glucosidase ranged from 3618% to 5271%.With the advantages of rapidness,high throughput and effectiveness,this method could screen α glucosidase inhibitors in Scutellaria baicalensis and provide valuable information for screening α glucosidase inhibitors from other traditional Chinese medicines. |
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