Preparation and Uncertainty Valuation of Naringenin Certified Reference Material
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KeyWord:naringenin  qualitative analysis  certified reference materials  uncertainty
LIU Jin-tao,LI Ling,DONG Jia-li,JIAO Bi-ning,SU Xue-su 1.College of Chemistry and Chemical Engineering,Southwest University;2.Laboratory of Quality and Safety Risk Assessment for Citrus Product,Ministry of Agriculture and Rural Affairs,Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences
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      A method for preparation and determination of naringenin certified reference material was established.Qualitative analysis of purchased samples was performed using infrared spectroscopy and nuclear magnetic resonance.The samples were purified by column chromatography and dried at 105 ℃ for 6 h,after mixing and dispensing,120 bottles of reference material candidates were obtained.Purity determination was carried out by differential scanning calorimetry and mass balance method.The mass balance method based on area normalization method of liquid chromatography,included detections on contents of moisture,inorganic impurities and residual solvents.Homogeneity and stability studies were validated by liquid chromatography,and uncertainty of the determination results was also analyzed.Results showed that the developed naringenin certified reference material had good homogeneity and stability,with a purity of 99.2%,an expanded uncertainty of 0.2%(k=2) and a validity period of 6 months.