Rapid Visual Screening of Illegally Added Salbutamol in Health Products Relieving Cough and Asthma by Colorimetric Method Based on Aptamer

KeyWord:salbutamol  aptamer  rapid visual screening method  illegal addition  health products relieving cough and asthma

Abstract:

      A rapid visual method based on aptamer sensor was established for screening of illegally added salbutamol£¨SAL£© in health products.The detection element of this sensor was the SAL-aptamer,and the affinity between SAL-aptamer and ssDNA-binding proteins£¨SSB£© acted as a ¡°switch¡± of the sensor.SAL-aptamer modified micromagnatic beads£¨MB£©£¨MB-aptamer£©,and horseradish peroxidase£¨HRP£© and SSB jointly modified silica microsphere£¨SiO2@SSB@HRP£© were prepared,respectively.Then MB-aptamer and SiO2@SSB@HRP were connected by the affinity between SAL-aptamer and SSB.SAL,as a key,triggered the ¡°switch¡± to result in disconnection between SSB and SAL-aptamer during the inspection process,and the free SiO2@SSB@HRP was separated by magnetic action to carry out a color reaction.Under the optimized conditions,the color reaction showed visible gradient variation according to the change of SAL concentration.The color reaction catalyzed by HRP was quantitatively characterized with an ultraviolet-visible spectrophotometer,there was a linear relationship for absorbance wtih SAL concentration in the range of 0.1-10 ¦Ìmol/L£¨r2=0.995 9£©,with a detection limit of 0.1 ¦Ìmol/L.The mean recoveries at three spiked levels were all in the range of 86.0%-106%,with relative standard deviations of 3.9%-12%.The method is simple,sensitive and rapid,and it could meet the requirements for the rapid screening of illegally added SAL in real samples.