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Research Advances in Separation and Enrichment Approaches for Phosphopeptides |
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DOI: |
KeyWord:phosphopeptide separation and enrichment mass spectrometry review |
Author | Institution |
LI Sha,WANG Lu,WANG Ying,CHEN Ping |
College of Life Science,Hunan Normal University |
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Abstract: |
Currently,the identification of phosphopeptides mainly depends on mass spectrometry,but the low abundance of phosphopeptide and the interference of non phosphopeptide affect the analysis and identification of mass spectrometry.Therefore,the enrichment of phosphopeptide before mass spectrometry is a prerequisite for further study of phosphoproteomics.This review summarizes the mechanisms and advantages or disadvantages of those traditional and new developed methods for phosphopeptides enrichment,including immobilized metal ion affinity chromatography(IMAC), metal oxide affinity chromatography(MOAC), strong cation/anion exchange chromatography(SCX/SAX), hydrophilic interaction chromatography(HILIC), ectrostatic repulsion-hydrophilic interaction chromatography(ERLIC), chemical derivatization, matrix assisted laser desorption/ionization(MALDI) plate specific enrichment method, and coupled methods. |
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