Determination of 19 Pesticides Residues in Eggs by Gas Chromatography with Solid Phase Extraction

KeyWord:gas chromatography  solid phase extraction  pesticides residues  QuEChERS

Abstract:

      A gas chromatographic(GC) method based on QuEChERS extraction salt package and solid phase extraction was established for the determination of 19 pesticides residues,i.e.bifenthrin,fenpropathrin,permethrin,cypermethrin,fenvalerate,deltamethrin,dichlorvos,methamidophos,acephate,diazinon,dimethoate,pirimiphos-methyl,chlorpyrifos,fenitrothion,profenofos,fipronil,fipronil-desulfinyl,fipronil sulfide and fipronil sulfone in eggs.The sample was extractd with acetonitrile and QuEChERS extraction salt package,and then purified with GCB adsorbent and C18 SPE.The analytes were separated on an Agilent DB-1701(30 m¡Á0.32 mm,0.25 ¦Ìm) polarity capillary column,then detected by GC with FPD detector and ECD detector,and quantified by external standard method.Results showed that there were good linear relationships for 19 pesticides in their respective concentration ranges,with correlation coefficients(r2) not less than 0.999 3.The recoveries at three spiked levels of low,meddle and high ranged from 78.6% to 105%,with relative standard deviations(n=6) of 1.6%-9ª±3%.The limits of detection(LOD,S/N=3) and limits of quantitation(LOQ,S/N=10) were in the ranges of 0.138-8.33 ¦Ìg/kg and 0.461-27.8 ¦Ìg/kg,respectively.With the advantages of simplicity,good separation,reliability and stability,the established method could meet the requirements for determination of pesticides multi residues in eggs.