Determination of 3-Benzoylurea Insecticides in Fruit Juice by High Performance Liquid Chromatography with ZIF-8@Fe3O4 Based Magnetic Solid phase Extraction

KeyWord:ZIF-8@Fe3O4  magnetic solid phase extraction  benzoylurea insecticides  fruit juice  high performance liquid chromatography

Abstract:

      A high performance liquid chromatography with magnetic zeolitic imidazolate framework (ZIF-8@Fe3O4) solid phase extraction was developed for the determination of three benzoylurea insecticides)(BUs),iª±e.penfluron,clofentezine and chlorfluazuron in fruit juice samples.The prepared ZIF-8@Fe3O4 sorbent was characterized by X-ray diffraction,Fourier transform infrared spectrometry,scanning electron microscopy and vibrating sample magnetometry.The extraction parameters were optimized,including pH value of solution,amount of sorbent,extraction time,eluting solvent type and volume(single elution volume¡Áelution times).Under the optimized conditions,there were good linear relationships in the range of 2.0-200 ng/mL for penfluron and clofentezine and 3.5-350 ng/mL for chlorfluazuron,with correlation coefficients(r) of 0.999.The limits of detection(S/N=3) for three analytes were in the range of 0.6-1.0 ng/mL,and the limits of quantitation(S/N=10) were 2.0-3ª±5 ng/mL.The recoveries for all targets at three spiked levels in fruit juice samples ranged from 82.7% to 98.6%,with intra-day and inter-day relative standard deviations(RSD) of 1.2%-6.1% and 1.3%-9.0%,respectively.The enhancement factors ranged from 43.4 to 48.1 for all the analytes when 10 mL juice sample was used as loading solution.The reusability of the fabricated ZIF-8@Fe3O4 adsorbent was investigated,and the RSD for recoveries of benzoylurea insecticides for 10 consecutive adsorption-desorption cycles in sample solutions were not more than 8.8%.The proposed method is sensitive and effective,and could be applied in the determination of benzoylurea insecticides in fruit juice samples.