Determination of Bisphenol Substances in Drug and Food Homologous Products by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry Combined with Accelerated Solvent Extraction and QuEChERS

KeyWord:accelerated solvent extraction(ASE£©  QuEChERS  ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS£©  bisphenol substances  drug and food homologous products

Abstract:

      A method was developed for the simultaneous determination of bisphenol substances in drug and food homologous products by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS£© combined with accelerated solvent extraction(ASE£© and QuEChERS.The samples were extracted at 80 ¡æ via two cycles of ASE,with acetonitrile-acetic acid-water(89¡Ã1¡Ã10,by volume£© for 6 min,then purified by QuEChERS.11 bisphenol substances were separated on an ACQUITY UPLC HSS T3 column(2.1 mm¡Á100 mm,1.8 ¦Ìm£© by gradient elution using methanol containing 0.1% formic acid and 5 mmol/L ammonium acetate containing 0.1% formic acid as mobile phases,then analyzed by MS/MS with electrospray ionization in positive and negative mode under multiple reaction monitoring(MRM£© mode,finally quantified by internal standard method.There were good linear relationships for 11 bisphenol substances in the concentration range of 0.5-50.0 ¦Ìg/L,with correlation coefficients(r2£© not less than 0.996 0.The limits of detection(LOD£© and limits of quantitation(LOQ£© for 11 bisphenol substances were in the ranges of 0.1-0.5 ¦Ìg/kg and 0.3-1.5 ¦Ìg/kg,respectively.The recoveries at three spiked levels ranged from 72.4% to 108% with relative standard deviations(RSD£© of 1.7%-15%.The proposed method was simple,rapid,high sensitive and reproducible,and it was suitable for the rapid determination of 11 bisphenol substances in drug and food homologous products.