Scientific PapersRapid Determination of Active Components in Extraction Process of Bupleuri Radix by Near Infrared Spectroscopy

KeyWord:near infrared spectroscopy(NIRS)  Bupleuri Radix  extraction process  active components  rapid analysis

Abstract:

      A near infrared spectroscopy(NIRS) was applied to the rapid and quantitative analysis of active components in the extraction process of Bupleuri Radix.Total of 126 extraction samples were selected,of which the contents of total flavonoids and polysaccharide were determined by ultraviolet-visible spectrophotometry,while the saikosaponin A and saikosaponin D were determined by high performance liquid chromatography(HPLC),and their spectra were collected in transmission mode.The quantitative calibration models for above four compounds were established by partial least squares(PLS),which were optimized by using different pretreatment methods,spectral bands and the number of principle components.Results indicated that there existed good fitting of the four quantitative calibration models between predicted values and the reference values,which exhibited high prediction accuracy with correlation coefficients in prediction sets(RP) greater than 0.9.The root mean square errors of prediction(RMSEP) were 3.46 ¦Ìg/mL,0.743 mg/mL,1.53 ¦Ìg/mL,0.406 ¦Ìg/mL for total flavonoids,polysaccharide,saikosaponin A and saikosaponin D,respectively,and the relative standard errors of prediction(RSEP) were 1.65%,8.28%,5.74% and 7.52%,respectively.All the results confirmed that the combination of NIRS and PLS could be successfully applied to the monitoring of active components in the extraction of Bupleuri Radix with the advantages of rapidness,accuracy,non-destruction and environmental friendly.