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| Simultaneous Determination of Four New Psychoactive Substances of Cathinone in Blood Samples by Ultra-high Performance Liquid Chromatography-Quadrupole-Time-of-flight Mass Spectrometry |
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| DOI： |
| KeyWord:ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UHPLC-QTOF MS/MS) new psychoactive substances of cathinone precipitated protein blood sample |
| Author | Institution |
| LIU Jing,HE Hong-yuan,NI Chun-fang,CAO Fang-qi,ZHAO Hai-bo,XU Lin |
1.School of Criminal Science and Technology,Chinese National Police University;2.Shanghai Key Laboratory of Crime Scene Evidence,Shanghai Public Security Bureau Material Certification Center;3.Shanghai Key Laboratory of Physical Evidence,Shanghai Institute of Criminal Science and Technology;4.Yingze Branch of Taiyuan Public Security Bureau |
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| Abstract: |
| An ultra high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-QTOF MS/MS) was established for the simultaneous determination of 4 kinds of new psychoactive substances of cathinone in blood samples,including 1-(3,4-methylenedioxyphenyl)-2-methylaminopropan-1-one(methylone),1-(3,4-methylenedioxyphenyl)-2 ethylaminopropan 1-one(ethylone),1-(4-chlorophenyl)-2-methylaminopropan-1-one(4 CMC) and 1-(4-chlorophenyl)-2-ethylaminopropan-1-one(4-CEC).0.5 mL blood sample was mixed with water and acetonitrile by a volume ratio of 5∶2∶13,vortex oscillated for 1 min,and centrifuged at 12 000 r/min for 15 min to precipitate the protein.The supernatant was separated on an Acquity UPLC-BEH C18 column(2.1 mm×100 mm,1.7 μm),with 0.1% formic acid-water(5 mmol/L ammonium acetate) and acetonitrile as mobile phases by gradient elution,and detected in positive ion［M+H］+ scanning under multiple reaction monitoring(MRM) mode.Results showed that there were good linear relationships for four target drugs in the concentration range of 5-500 ng/mL,with the limits of detection and limits of quantitation of 2 ng/mL and 5 ng/mL,respectively.The recoveries ranged from 87.3% to 111% with the intra day precision and inter day precision not more than 8.1% and 8.6%,respectively.The method could be used for the simultaneous detection of 4 new psychoactive substances of cathinone in blood samples to meet the needs of actual inspection work. |
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