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Research on Construction and Application of a Dual-recognition Photoelectrochemical Sensor for Chlorpyrifos Based on BiOI Nanosheets |
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DOI: |
KeyWord:BiOI nanosheets chlorpyrifos molecular imprinting acetylcholinesterase(AChE) water sample photoelectrochemical sensor |
Author | Institution |
JIN Dang-qin,GONG Ai-qin,WANG Yuan-you,YONG Da-ming,SUN Qiang,ZHU Wen-jun,SUN Yan-yan |
Chemical Engineering Institute, Yangzhou Polytechnic Institute, Yangzhou , China |
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Abstract: |
A chlorpyrifos molecularly imprinted polymer membrane was synthesized by electropolymerization, with BiOI nanosheets as the carrier, chlorpyrifos as the template molecule, p-mercaptoaniline as the functional monomer and glutaraldehyde as the crosslinking agent. Based on this, a dual-recognition photoelectrochemical sensor for chlorpyrifos(CPF)was constructed by immobilizing acetylcholinesterase(AChE) on the surface of the imprinted membrane. The current-time method (I-t) was used to detect chlorpyrifos in water samples.Acetylthiocholine chloride(ATCl) concentration and pH value were optimized. Results showed that,under the optimal experimental conditions(1.0 mmol/L ATCl,pH 7.0), the peak currents and chlorpyrifos concentrations were linear in the ranges of 0.02-1.0 nmol/L and 20.0-200.0 nmol/L. The linear equations were inhibition rate(%) = 49.253 CCPF + 9.433(r2 = 0.998 6) and inhibition rate(%) = 0.193 CCPF + 57.599(r2 = 0.999 1), respectively. The detection limit(S/N = 3) was 0.005 nmol/L. The sensor had a good selective recognition ability, reproducibility(RSD = 4.2%) and stability for chlorpyrifos. The method was successfully applied to the detection of trace chlorpyrifos in water samples, with spiked recoveries of 96.1% -108%, and RSDs of 2.8%-4.2%. |
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