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| Determination and Statistical Analysis of Ketamine and Its Metabolites in Human Hair by Ultra-performance Liquid Chromatography Coupled with Mass Spectrometry |
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| DOI： |
| KeyWord:hair UPLC－MS/MS ketamine and its metabolites content statistics |
| Author | Institution |
| HOU Wei， ZHANG Lei-ping，WANG Ji-fen，WEI Chun-ming，LU Liang，ZHONG Li-jing，LIU Hao-tian |
1. School of Investigation，People′s Public Security University of China，Beijing ，China; 2. Institute of Forensic Science，Ministry of Public Security，Beijing ，China |
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| Abstract: |
| An ultra-performance liquid chromatography－mass spectrometry（UPLC－MS/MS） with high kinetic energy grinding was established for the rapid detection of ketamine and its metabolites in hair．The effects of cleaning procedures and extraction methods on the detection of ketamine and its metabolites were investigated，respectively，and the best experimental scheme was determined．50 mg hair was washed twice with 0.1% sodium lauryl sulphate，ultrapure water and acetone．Then 20 mg of the washed hair and 1 mL methanol were placed in a special grinding bottle，and mixed in an oscillating manner．The hair was ground for 3 min at a frequency of 3 000 r/min by the grinding machine．The grinding fluid was ultrasonically disposed for 2 hours，then detected after centrifugation and filtration．There were good linear relationships for contents of ketamine，norketamine and hydroxynorketamine in hair in the range of 0.01－5.0，0.01－5.0，0.05－5.0 ng/mg，with their correlation coefficients higher than 0.99．The extraction recoveries ranged from 86.7% to 106%，and the matrix effects were between 88.1% and 99.6% for all the analytes．The intra-day and inter-day precisions were in the range of 0.72%－4.1% and 1.9%－6.3%，respectively．Meanwhile，50 hair samples of drug addicts were tested in this way．The content data of ketamine，norketamine and droxynorketamine in positive hair were obtained and analyzed．The content distribution of ketamine and its metabolites and the relationship between the contents of ketamine and its metabolites in hair were determined by data analysis．The minimum content of ketamine was 0.30 ng/mg．The content of droxynorketamine was similar to ketamine while the content ratio between norketamine and ketamine ranged from 0.05 to 0.60．These analyses and conclusions could provide a theoretical support and a method reference for the drug-related cases． |
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