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Detection of Rutin Using an Enzyme-free and Label-free Fluorescent Probe Based on Gold Nanoclusters-Bovine Serum Albumin |
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DOI: |
KeyWord:rutin gold nanoclusters fluorescent probe enzyme-free and label-free bovine serum albumin |
Author | Institution |
ZHOU Qiu-min,LAI Yan-qiong,LI Qiu-yang,ZHANG Yan-li,WANG Hong-bin,YANG Wen-rong,PANG Peng-fei |
1. School of Chemistry and Environment, Yunnan Minzu University, Kunming , China; 2. School of Life and Environmental Sciences, Deakin University, Geelong VIC 3217, Australia |
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Abstract: |
An enzyme-free and label-free fluorescence sensing method for rutin detection was developed based on gold nanoclusters-bovine serum albumin(AuBCs-BSA) fluorescent probe prepared with bovine serum albumin(BSA) as template.The morphology and optical property of the prepared AuNCs-BSA were characterized by transmission electron microscopy(TEM),ultraviolet-visible spectroscopy(UV-Vis) and fluorescent spectroscopy(FL).The experimental results showed that fluorescence of AuNCs-BSA system was quenched in the presence of rutin as the affinity between BSA and rutin was stronger than that between BSA and AuNCs,resulting in the release of AuNCs from BSA.Under the optimized experimental conditions,the linear range of AuNCs-BSA fluorescent probe for rutin detection was 0-60 μmol/L with a detection limit(S/N = 3) of 0.63 μmol/L.The spiked recoveries of real sample were 99.0%-103% with relative standard deviations less than 3%.The developed fluorescent probe has the advantages of simple preparation,no labeling and high sensitivity,and provides a simple,reliable and effective method for the determination of rutin in practical samples. |
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