Non-targeted Screening of Nonvolatile Migration Compounds in Recycled PET Based on Whole Chain and Their Identification of Characteristic Risk Markers

KeyWord:recycled PET  food contact materials  ultrahigh performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry  MS£­DIAL  risk assessment

Abstract:

      Based on the whole chain of recycled polyethylene terephthalate£¨recycled PET£¬rPET£©£¬potential migrants in recycled flakes£¬pellets£¬preforms and bottles/migrants in the food simulation solution were untargeted screened by ultrahigh performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry£¨UPLC£­Q£­TOF MS£©£®Thirty kinds of intentionally or non-intentionally added substances including PET oligomers£¬lubricants and rinsing agents were screened out£®MS£­DIAL£¨Mass spectrometry£­data independent analysis software£© was employed to screen the markers of rPET products in different production stages£®The results showed that new PET oligomers were formed during the processing from pellet to preform£®Alcohol ethoxylate was detected in the flakes of rPET due to the clean process£®Comparing the relative content of PET oligomers£¬it was found that the relative content of cyclic oligomers in rPET was higher than that of linear oligomers£¬while the relative content of the second series of cyclic dimers was the highest£®The relative content of several kinds of oligomers and lubricants increased during rPET processing£®Finally£¬the TEST£¨Toxicity estimation software tool£© was used to predict the rat oral acute toxicity£¬developmental toxicity and Ames mutagenicity of potential migrants/migrants£®And the classification of potential migrants/migrants by Cramer rules was performed by Toxtree software. It was found that PET cyclic oligomers and amide lubricants were all classified as Cramer ¢ó£¬which was more toxic than linear oligomers£¬suggesting that attention should be paid during the rPET recycling process£®