Determination of Migration of 9 Phenols in Food Contact Materials by High Performance Liquid Chromatography

KeyWord:phenols  migration  food contact materials  high performance liquid chromatography £¨HPLC£©  fluorescence detector

Abstract:

      A high performance liquid chromatographic£¨HPLC£© method was established for the simultaneous determination of 9 phenols such as phenol£¬methyl-phenols and xylenols in food simulants. The sample was separated on an Agilent ZORBAX SB£­Aq column by gradient elution£¬using methanol and water as mobile phase at a flow rate of 1.0 mL/min£¬and finally determined by fluorescence detection at an excitation wavelength of 214 nm and an emission wavelength of 315 nm£®Meanwhile£¬the extraction method for 9 phenols in the fat-contained simulant was optimized£¬and the performances of the method including linearity£¬spiked recovery and precision were studied£®Results demonstrated that 67% methanol aqueous solution was the best extraction solvent for the 9 phenols in fat simulants£®The results also showed that there were good linear relationships for 9 phenols in all food simulants studied£¬with correlation coefficients£¨r2£© higher than 0.999£®The recoveries for 9 phenols at 3 spiked levels in all food simulants ranged from 90.7% to 99.8%£¬with relative standard deviations £¨RSD£© all below 6.0%£®The limits of quantitation£¨LOQs£© were in the ranges of 0.07£­0.10 mg/L for aqueous simulants£¬and 0.15£­0.20 mg/kg for fat simulants. This method was successfully applied to the migration determination of 9 phenols released from 11 plastics articles£¨PC£© and 15 coatings intended to contact with food£®The method was sensitive and accurate£¬and was suitable for the determination of 9 phenols in food simulants.