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| Rapid Detection of Kanamycin Based on Aptamer Colloid Gold Lateral Flow Test Strip |
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| DOI： |
| KeyWord:gold nanoparticles lateral flow test strip kanamycin aptamer |
| Author | Institution |
| CHANG Rui，CAI Yi-na，WANG Zhou-ping，PENG Chi-fang |
1. State Key Laboratory of Food Science and Technology，Jiangnan University，Wuxi ，China; 2. School of Food Science and Technology，Jiangnan University，Wuxi ，China; 3. Food Inspection and Quarantine Centre，Shenzhen Customs，Shenzhen ，China |
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| Abstract: |
| In this paper，a new type of colloidal gold lateral flow test strip（AuNPs@polyA－DNA） was fabricated based on aptamer recognition of kanamycin molecule and non-thiolated DNA modified colloidal gold nanoprobe. Effects of the strip components on color development were investigated，including concentration of aptamer，proportion of streptavidin（SA） to Biotin－DNAT，concentration of SA－Biotin－DNAT，conjugate reaction time and temperature．The optimized experimental conditions were as follows：Running buffer：4xSSC（0.5% Tween 20），molar ratio of SA to Biotin－DNAT：1∶6，concentartion of SA－Biotin－DNAT conjugate sprayed at T line：4 μmol/L，concentartion of aptamer：10 nmol/L，incubation of aptamer： ambient temperature for 20 min．The results demonstrated that 25 ng/mL kanamycin could be discriminated with the naked eyes．The quantitative determination of kanamycin in the range of 5.0－125 ng/mL could be carried out by a colloidal gold reader，with a detection limit of 1.5 ng/mL．The test strip was applied to the determination of kanamycin in honey，with recoveries of 95.1%－105% and relative standards deviation（RSD） of 3.4%－8.5%．The strip could be used for the detection of kanamycin in real samples with the advantages of high sensitivity，high specificity，simple structure and high repeatability． |
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